penicillin-binding protein 2d

class A penicillin-binding protein 2d

BSU37510

BSU_37510

647

691

1941

2076

bifunctional glucosyltransferase/ transpeptidase

bifunctional glucosyltransferase/ transpeptidase, synthesis of spore peptidoglycan

BSU37510

BSU_37510

cell membrane (according to Swiss-Prot)

prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 Link to a summary]

cell membrane, the major part of the protein is exposed to the outside (according to UniProt)

prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 Link to a summary]

16497325, 11567005, 10767540, 20525796, 15758244

16497325, 11567005, 10767540, 20525796, 15758244, 19458048, 29456081

a strain lacking all four class A [SW|penicillin-binding proteins] ([[gene|ponA]] [[gene|pbpD]] [[gene|pbpF]] [[gene|pbpG]]) is severely inhibited for L-form switching in the presence of D-cycloserine [pubmed|29456081]

synthesis of spore peptidoglycan (with [[protein|PbpF]]) [pubmed|11567005]

[GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)]_(n)-diphospho-di-trans,octa-cis-undecaprenol + β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol --> [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)]_(n+1)-diphospho-di-trans-octa-cis-undecaprenol + di-trans,octa-cis-undecaprenyl diphosphate + H⁺ (according to UniProt)

Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine (according to UniProt)

N-terminal part: [SW|glycosyltransferase 51 family] (according to UniProt)

C-terminal part: [SW|transpeptidase family] (according to UniProt)

[[protein|PbpD]], [[protein|PbpF]], [[protein|PonA]]

[PDB|3VMA] (PBP1B from E. coli, 29% identity) [pubmed|19458048]